Protein NMR Techniques / Edition 2

by A. Kristina Downing

Overview

When I was asked to edit the second edition of Protein NMR Techniques, my first thought was that the time was ripe for a new edition. The past several years have seen a surge in the development of novel methods that are truly revolutionizing our ability to characterize biological macromolecules in terms of speed, accuracy, and size limitations. I was particularly excited at the prospect of making these techniques accessible to all NMR labs and for the opportunity to ask the experts to divulge their hints and tips and to write, practically, about the methods. I commissioned 19 chapters with wide scope for Protein NMR Techniques, and the volume has been organized with numerous themes in mind. Chapters 1 and 2 deal with recombinant protein expression using two organisms, E. coli and P. pastoris, that can produce high yields of isotopically labeled protein at a reasonable cost. Staying with the idea of isotopic labeling, Chapter 3 describes methods for perdeuteration and site-specific protonation and is the first of several chapters in the book that is relevant to studies of higher molecular weight systems. A different, but equally powerful, method that uses molecular biology to “edit” the spectrum of a large molecule using segmental labeling is presented in Chapter 4. Having successfully produced a high molecular weight target for study, the next logical step is data acquisition. Hence, the final chapter on this theme, Chapter 5, describes TROSY methods for stru- ural studies.

Product Details

ISBN-13:	9781617374494
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #278
Edition description:	Softcover reprint of hardcover 2nd ed. 2004
Pages:	487
Product dimensions:	5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Screening and Optimizing Protein Production in E. coli.- Isotopic Labeling of Recombinant Proteins from the Methylotrophic Yeast Pichia pastoris.- Perdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight Proteins.- Segmental Isotopic Labeling for Structural Biological Applications of NMR.- TROSY-Based Correlation and NOE Spectroscopy for NMR Structural Studies of Large Proteins.- Media for Studies of Partially Aligned States.- Residual Dipolar Couplings in Protein Structure Determination.- Projection Angle Restraints for Studying Structure and Dynamics of Biomolecules.- Characterizing Domain Interfaces by NMR.- Characterization of the Overall Rotational Diffusion of a Protein From 15N Relaxation Measurements and Hydrodynamic Calculations.- TROSY-Based NMR Experiments for the Study of Macromolecular Dynamics and Hydrogen Bonding.- Measurement of Intermediate Exchange Phenomena.- NMR Studies of Partially Folded Molten-Globule States.- Structure Determination of Protein Complexes by NMR.- NMR Studies of Protein-Nucleic Acid Interactions.- Using NMRView to Visualize and Analyze the NMR Spectra of Macromolecules.- Automated NMR Structure Calculation With CYANA.- NOE Assignment With ARIA 2.0.- Membrane Protein Structure Determination Using Solid-State NMR.

Editorial Reviews

From reviews of the first edition...

"...includes many good practical details which are so often omitted and which workers new to the field have to rediscover..." -Analyst

"...an excellent text... a very useful compilation...The limitations and power of the approaches are well presented and should help in experimental design very considerably." -Journal of Medicinal Chemistry

"...a collection of excellent reviews that will form a useful contribution to the bookshelf of any NMR lab." -Trends in Biotechnology

"...an excellent introduction...Readers will be left with a good understanding of the application of NMR to the study of proteins. " -Quarterly Review of Biology

From the Publisher