Reconstitutions of Transporters, Receptors, and Pathological States

by Efraim Racker

Overview

Reconstitutions of Transporters, Receptors, and Pathological States presents lectures about the reconstitutions of transporters, receptors, and pathological states. The book discusses the principles and strategies of the resolution and reconstitution of soluble pathways and membrane complexes; and lessons in the resolution and reconstitution after the natural structure of the membrane has been destroyed. The text then describes the analyses of reconstituted vesicles; the ATP synthetase of oxidative phosphorylation; and the ?1?2 pumps of plasma membranes. The ATP-driven ion pumps in organelles, microorganisms, and plants; the proton motive force generators, electron transport chains, and bacteriorhodopsin; and facilitated diffusion, symporters, and antiporters are also considered. The book further tackles plasma membrane receptors, as well as the reconstitutions of pathological states. Biochemists, molecular biologists, and cell biologists will find the book invaluable.

Product Details

ISBN-13:	9781483214238
Publisher:	Elsevier Science
Publication date:	01/01/1985
Sold by:	Barnes & Noble
Format:	eBook
Pages:	288
File size:	3 MB

Table of Contents

PrefaceLessonsAbbreviationsLecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies I. Reconstitution of Soluble Pathways II. Resolution and Reconstitution of Membrane ComplexesLecture 2 Methods of Resolution and Reconstitution I. Solubilization and Purification of Membrane Proteins II. Purification of Membrane Proteins III. Methods of ReconstitutionLecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed I. What Are the Protein Components of the System and What Are Their Functions II. What Are the Phospholipid Components and What Are Their Functions III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It IV. How Do We Measure the Extent of Scrambling during Reconstitution VI ContentsLecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles I. Analysis of Reconstituted Vesicles II. Pitfalls and Recommended CautionsLecture 5 The ATP Synthetase of Oxidative Phosphorylation I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1) II. The Stalk, OSCP, and F6 III. The Hydrophobie Sector IV. Mechanism of Action of F1Lecture 6 The E1E2 Pumps of Plasma Membranes I. The Na+,K+ Pump and Na+,K+-ATPase II. The Ca2+ Pump and Ca2+-ATPase III. The H+,K+-ATPase of the Gastric MucosaLecture 7 I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles II. ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants III. The ATP-Driven H+ Fluxes in Organelles ATP-Driven Ion Pumps of Microorganisms and PlantsLecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin I. Reconstitution of the Mitochondrial Electron Transport Chain II. Reconstitution of Photosynthetic Electron Transport Pathways III. Bacteriorhodopsin IV. Halorhodopsin and a Bacterial Na+ Pump Lecture 9 Facilitation Diffusion, Symporters, and Antiporters I. Transporters of Plasma Membranes II. Transporters of Mitochondria III. Transporters of Other Organelles Lecture 10 Plasma Membrane Receptors I. RGC Receptors II. Polypeptide Signal Receptors III. Channel Receptors IV. Transport Receptors V. Drug and Toxin ReceptorsLecture 11 Reconstitution of Pathological States I. About the Artificiality of Cancer Research II. Two Approaches to Cancer Research III. The Scenic Route from ABC to XLecture 12 Glimpses into the Future of Reconstitutions (Hypotheses, Speculations, and Fantasies) I. Methods of Reconstitution II. Orientation-Directed Reconstitution and Co-reconstitutions III. Mechanisms and Regulations IV. Incorporations of Cellular Components into Cells V. Reconstitution of Organelles, Cells, Organs, etc VI. Reconstitution of Pathological States BibliographyIndex