Spectroscopic Methods and Analyses: NMR, Mass Spectrometry, and Metalloprotein Techniques / Edition 1

by Christopher Jones, Barbara Mulloy, Adrian H. Thomas

Overview

The three volumes in Methods in Molecular Biology covering Physical Methods of Analysis (vol. 1, Spectroscopic Methods and Analyses: NMR, Mass Spectrometry, and Metalloprotein Techniques; vol. 2, Optical Spectroscopy and Macroscopic Techniques; vol. 3, Cryst- lographic Methods and Techniques) differ from others in this series in several ways. Each volume covers a group of techniques for the char- terization of biological molecules and their interactions that involve the application of modern techniques of physical chemistry. These techniques by and large do not lend themselves to the "hands-on" approach and cannot usually be carried out by the molecular biologist alone, but most often require collaboration with a specialist. The biologist or biochemist contemplating such a collaboration may feel somewhat at a distance from the experimental work and further isolated by the use of the jargons of analytical and physical chemistry. Physical methods have been used in molecular biology from the earliest days, from simple applications of optical spectroscopy to the complexity of X-ray crystallography, and the full range of these me- ods will be covered over the three volumes. The methods dealt with in this first volume have largely developed from beginnings in small molecule chemistry to the point where they play a valuable role in the characterization of biological macromolecules.

Product Details

ISBN-13:	9780896032156
Publisher:	Springer-Verlag New York, LLC
Publication date:	05/23/1993
Series:	Methods in Molecular Biology , #17
Edition description:	1993
Pages:	395
Product dimensions:	5.83(w) x 8.27(h) x 0.04(d)

Table of Contents

Nmr Spectroscopy.- to Nuclear Magnetic Resonance.- Structural Studies of Proteins in Solution Using Proton Nuclear Magnetic Resonance.- Peptide Structure Determination by NMR.- High-Resolution NMR of DNA and Drug-DNA Interactions.- Structural Characterization of the Carbohydrate Moieties of Glycoproteins by High-Resolution 1H-NMR Spectroscopy.- The Application of Nuclear Magnetic Resonance to Structural Studies of Polysaccharides.- Dynamic and Exchange Processes in Macromolecules Studied by NMR Spectroscopy.- Nmr Spectroscopy.- to Mass Spectrometry.- Laser Desorption Ionization Mass Spectrometry of Bioorganic Molecules.- 252-Californium Plasma Desorption Time-of-Flight Mass Spectrometry of Peptides and Proteins.- Fast Atom Bombardment Mass Spectrometry of Peptides.- Tandem Mass Spectrometry.- Nmr Spectroscopy.- Mössbauer Spectroscopy.- Electron Paramagnetic Resonance Spectroscopy of Metalloproteins.- Resonance Raman Spectroscopy of Metalloproteins Using CW Laser Excitation.- The Application of X-Ray Absorption Spectroscopy to Characterize Metal Centers in Proteins.

Editorial Reviews

The main strength of this volume...is the extensive attention paid to the pragmatic and mundane issues that are only gleaned with difficulty from the literature, yet are absolutely critical for success. Those contemplating initiating studies employing these physical methods would be well served by consulting this manual. - Modern Pathology

From the Publisher

Although spectroscopic methods are of great use to biological scientists, many researchers lack a specialist's knowledge of these techniques. This guide provides hands-on information on these research tools, emphasizing practical aspects such as amounts and purity of required samples, limitations and advantages of the technique, and interpretation of the results, thus enabling molecular biologists--either independently or with the help of a specialist--to evaluate the usefulness of a technique, to plan an original program, and to gain a realistic idea of the resources needed. Plastic comb-binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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