Target Discovery and Validation Reviews and Protocols: Emerging Molecular Targets and Treatment Options,Volume 2 / Edition 1

by Mouldy Sioud

Overview

Target Discovery and Validation Reviews and Prools, Volumes 1 and 2 review the most progressive and current methods for drug target discovery and validation. These volumes explore how recent improvement in understanding the molecular mechanisms of human pathology is impacting drug target discovery in the laboratory and in real therapeutics, specifically for cancers and autoimmune disorders.

Volume 1 focuses on novel and innovative techniques, and presents the most up-to-date prools available for maximizing the likelihood of achieving target-selective inhibition in vivo while minimizing side effects. The profound impact of genomics, proteomics and bioinformatics on target discovery is explored, and specific attention is given to the role of transgenic and knockout animals in functional genomics and target validation. Cancer researchers will find tremendous value in the molecular classification of breast cancers and the review of prools for tumor antigens and cancer vaccines. The methods and prools collected here, all reviewed by leading scientists and clinicians, present the practical details necessary for translating the enormous discovery potential of the genome into real therapeutic products.

Volume 2 collects all the practical details required for efficient translation of discovered targets into real pharmaceutical drugs. Specific targets in cancers and autoimmunity are described and the potential of using siRNAs, antisense oligonucleotides and RNA aptamers in patients is reviewed. This volume explores the tremendous impact of the application of genotyping and gene expression profiling on the future of healthcare, and presents cutting-edge prools to aid in bringing agents against specific targets closer to application in the clinic.

Collectively, these volumes provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application. For researchers, an understanding of available methods aids in the creation of innovative experiments in the laboratory, and the successful translation of target discovery to real therapeutics.

Product Details

ISBN-13:	9781588298904
Publisher:	Springer-Verlag New York, LLC
Publication date:	02/05/2007
Series:	Methods in Molecular Biology , #361
Edition description:	2007
Pages:	345
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Druggable Signaling Proteins.- DNA Methylation and Histone Modifications in Patients With Cancer.- Wnt Signaling as a Therapeutic Target for Cancer.- The NG2/HMP Proteoglycan as a Cancer Therapeutic Target.- Heterotrimeric G Proteins and Disease.- High-Mobility Group Box-1 Isoforms as Potential Therapeutic Targets in Sepsis.- Antisense Oligonucleotides.- Nucleic Acid-Based Aptamers as Promising Therapeutics in Neoplastic Diseases.- Guidelines for the Selection of Effective Short-Interfering RNA Sequences for Functional Genomics.- Suppression of Apoptosis in the Liver by Systemic and Local Delivery of Small-Interfering RNAs.- Target Validation Using RNA Interference in Solid Tumors.- Validation of Telomerase and Survivin as Anticancer Therapeutic Targets Using Ribozymes and Small-Interfering RNAs.- Collagen-Induced Arthritis in Mice.- Novel Opportunities for Therapeutic Targeting in Systemic Autoimmune Diseases.- Considerations for Target Validation and Industrial Approaches.- Regulatory RNAs.- Treatment Options and Individualized Medicine.

Editorial Reviews

Reviewer: Bao Ting Zhu, PhD (University of Kansas Medical Center)
Description: This volume in the Methods in Molecular Biology series reports on some of the protocols relevant to emerging molecular targets and treatment options. It focuses on some of the practical details of conducting translational research aimed at converting discovered targets into pharmaceutical drugs. Discussed at considerable length are targets in cancers and autoimmunity, the use of siRNA, and the use of antisense oligonucleotides and RNA aptamers in patients. Some chapters also explore the application of genotyping and gene expression profiling as a potential future tool.
Purpose: The purpose is to provide an updated description and discussion of the protocols relevant to developing emerging molecular targets as treatment options.
Audience: This book is of value to basic science researchers as well as clinicians for a better understanding of some of the methods involved in this new area of research.
Features: One of the book's notable features is the way it effectively combines the theoretical aspects of topics with their clinical applications. Clinical examples are often provided to help readers better understand the potential usefulness of these methods.
Assessment: This book provides an effective, concise review of some of the state-of-the-art methods that can be used for therapeutic/drug target identification. Examples illustrate their potential clinical applications. The book will be helpful to basic biomedical scientists as well as to clinicians who want a quick overview of this area of research and its recent developments. The book also provides some useful methodological guidelines and examples for researchers new to this field who are considering embarking on a venture into this area.

Doody's Review Service

From the reviews:

"...provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical applications." -Anticancer Research

"Review current methods for drug target discovery and validation. … the chapters are generally well written and clear, and provide extensive references to the current literature. Overall … provide useful overviews that would provide entry into this field. Novice and more experienced researchers … would find useful information … ." (Jonathan B. Chaires, ChemMedChem, Issue 3, 2008)

From the Publisher

Reviewer: Bao Ting Zhu, PhD(University of Kansas Medical Center)
Description: This volume in the Methods in Molecular Biology series reports on some of the protocols relevant to emerging molecular targets and treatment options. It focuses on some of the practical details of conducting translational research aimed at converting discovered targets into pharmaceutical drugs. Discussed at considerable length are targets in cancers and autoimmunity, the use of siRNA, and the use of antisense oligonucleotides and RNA aptamers in patients. Some chapters also explore the application of genotyping and gene expression profiling as a potential future tool.
Purpose: The purpose is to provide an updated description and discussion of the protocols relevant to developing emerging molecular targets as treatment options.
Audience: This book is of value to basic science researchers as well as clinicians for a better understanding of some of the methods involved in this new area of research.
Features: One of the book's notable features is the way it effectively combines the theoretical aspects of topics with their clinical applications. Clinical examples are often provided to help readers better understand the potential usefulness of these methods.
Assessment: This book provides an effective, concise review of some of the state-of-the-art methods that can be used for therapeutic/drug target identification. Examples illustrate their potential clinical applications. The book will be helpful to basic biomedical scientists as well as to clinicians who want a quick overview of this area of research and its recent developments. The book also provides some useful methodological guidelines and examples for researchers new to this field who are considering embarking on a venture into this area.