The Nucleic Acid Protocols Handbook / Edition 1

by Ralph Rapley

Overview

The Nucleic Acid Prools Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology™ series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Prools Handbook, Ralph Rapley has brought together thewidest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect.

Product Details

ISBN-13:	9780896038417
Publisher:	Springer-Verlag New York, LLC
Publication date:	03/24/2000
Series:	Springer Protocols Handbooks
Edition description:	2000
Pages:	478
Product dimensions:	7.01(w) x 10.00(h) x 0.08(d)

Table of Contents

Nucleic Acid Extraction.- Basic Separation and Analysis of DNA.- Probe Design, Synthesis, and Labeling.- RNA Analysis Techniques.- Gene Library Construction and Screening.- DNA Sequencing.- Basic Polymerase Chain Reaction Methods.- Analyzing Genes, Mutations, and Protein Interactions.- mutagenesis, transcription, and Translation In Vitro.- Gene Localization, Mapping In Situ, and Bioinformatics.

Editorial Reviews

"... the techniques are presented in minute details and in a "cookbook-standardized manner"... The personal notes inserted by each contributor provide assurance that the techniques would work in any laboratory... Having all of those prools updated and tested in practice and bound in one volume will be attractive to many scientists who deal with molecular biology techniques."- Modern Pathology

"There were over 190 authors involved in writing the prools, ...each prool is presented in outline format an follows a common numbering scheme, making it easy to find relevant sections....Many of the prools include tables of data or figures, including drawings or photographs of gels...One of the most useful sections in each prool lists notes, which elucidate possible pitfalls, provide troubleshooting tips, or give alternate methods or explanations for steps. This very practical information makes successful duplication of the procedure much more likely than when attemptingto follow the often less detailed or less flexible descriptions given in journal articles....this handbook would be extremely useful as a reference in most university libraries."-E-Streams

"...it covers practically all of the very important and large field of nucleic acid research. The volume summarizes, in 10 parts, recent advances in the isolation , analysis and manipulation of nucleic acids. ...The chapters were contributed by authors from leading laboratories in the field all over the world....The book provides the reader with a large set of ready-to use prools needed to undertake complex molecular biology experimental project on a large spectrum of biological models involving isolation and analysis of nucleic acids. It is thus an almost indispensable handbook for anybody working in the field of molecular biology and genetics."-Folia Microbiologica

"...extremely useful as a reference in most university libraries." - E-Streams

"...comprehensiveand detailed...this is an set of recipes for the genetic kitchen." -The Biologist

From the Publisher