Tissue Proteomics: Pathways, Biomarkers, and Drug Discovery / Edition 1

by Brian Liu, Joshua R. Ehrlich

Overview

With the sequencing of the human genome complete, the field of proteomics, still in its early stages, has become an important and informative field of biomedical research. Many of the prools and techniques commonly employed by proteomics researchers have been refined over the past several years, while at the same time, new and innovative methods have also been developed. Significantly, many of the prools used in the study of the human proteome have been developed to examine proteins derived specifically from cell lines, body fluids or human tissues. As a consequence, not all prools are compatible with the full-range of protein sources commonly used in the laboratory. The current volume on Tissue Proteomics seeks to bring together a number of useful and innovative prools developed particularly for the proteomic profiling of human tissues. The first two prools presented in this work focus on high-throughput g- based techniques. Chapter 1 describes 2-D DIGE, a method used to examine the entire proteome within discreet pI fractions, whereas Chapter 2 details several complementary techniques for the specific analysis of glycoproteins found in tissue samples. The next chapters, Chapters 3 and 4, focus on SELDI-MS and MALDI-TOF, two techniques that make use of mass spectrometry for the characterization and identification of proteins. These techniques may be used alone or in combination with other proteomic methods, such gel-based assays, where mass spectrometry is useful for additional characterization and identification of proteins.

Product Details

ISBN-13:	9781588296795
Publisher:	Springer-Verlag New York, LLC
Publication date:	01/31/2008
Series:	Methods in Molecular Biology , #441
Edition description:	2008
Pages:	226
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Preface.- Contributors.- 1 Capabilities Using 2-D DIGE in Proteomics Research: The New Gold Standard for 2-D Gel ElectrophoresisChristine R. Rozanas and Stacey M. Loyland.- 2 Protein Carbohydrate Analysis: Gel-Based Staining, Liquid Chromatography, Mass Spectrometry, and Microarray ScreeningInjae Shin, Alina D. Zamfir, and Bin Ye.- 3 Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of Body FluidsAudrey Gagnon, Qian Shi, and Bin Ye.- 4 A Novel Approach Using MALDI-TOF/TOF Mass Spectrometry and Prestructured Sample Supports (AnchorChip Technology) for Proteomic Profiling and Protein IdentificationSau-Mei Leung and Rebecca L. Pitts.- 5 Automated Laser Capture Microdissection for Tissue ProteomicsAdrianna S. Rodriguez, Benjamin H. Espina, Virginia Espina, and Lance A. Liotta.- 6 Tissue Microarrays: An OverviewRajiv Dhir.- 7 Frozen Protein ArraysStephen M. Hewitt and Robert A. Star.- 8 Reverse Phase Protein Microarrays for Theranostics and Patient-Tailored TherapyVirginia Espina, Julia Wulfkuhle, Valerie S. Calvert, Lance A. Liotta, and Emanuel F. Petricoin III.- 9 Antibody Arrays for Determination of Relative Protein AbundancesGrigoriy S. Chaga.- 10 SH2 Domain-Based Tyrosine Phosphorylation ArrayXin Jiang, Lesile Roth, Stephanie Han, and Xianqiang Li.- 11 Immunoregulomics: A Serum Autoantibody-Based Platform for Transcription Factor ProfilingOliver W. Tassinari, Margarita Aponte, Robert J. Caiazzo Jr., and Brian C.-S. Liu.- 12 The “Reverse Capture” Autoantibody Microarray: An Innovative Approach to Profiling the Autoantibody Response to Tissue-Derived Native AntigensJoshua R. Ehrlich, Liangdan Tang, Robert J. Caiazzo Jr., Daniel W. Cramer, Shu-Kay Ng, Shu-Wing Ng, and Brian C.-S. Liu.- 13 Biorepository Standards andProols for Collecting, Processing, and Storing Human TissuesDean Troyer.