Z-DNA: Methods and Protocols

by Kyeong Kyu Kim (Editor), Vinod Kumar Subramani (Editor)

Overview

This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and genome-targeting methods and genome-wide approaches, Z-RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Z-DNA: Methods and Prools serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z.

Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This is an open access book.

Product Details

ISBN-13:	9781071630839
Publisher:	Springer US
Publication date:	03/10/2023
Series:	Methods in Molecular Biology , #2651
Edition description:	2023
Pages:	332
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

The Origin of Left-Handed Poly[d(G-C)].- Characterization of Z-DNA Using Circular Dichroism.- Characterization of Z-DNA by Infrared Spectroscopy.- Crystallization of Z-DNA in Complex with Chemical and Z-DNA Binding Z-Alpha Protein.- NMR Titration Studies in Z-DNA Dynamics.- Single-Molecule Methods to Study Z-DNA Mechanics and Dynamics.- BZ Junctions and Its Application as Probe (2AP) to Detect Z-DNA Formation and Its Effector.- Oligonucleotide Containing 8-Trifluoromethyl-2’-Deoxy-Guanosine as a Z-DNA Probe.- Chiroptical Properties of Z-DNA Using Ionic Porphyrins and Metalloporphyrins.- Construction of a Z-DNA Specific Recombinant Nuclease Zαα-FOK for Conformation Studies.- Human Heme Oxygenase-1 Promoter Activity is Mediated by Z-DNA Formation.- ChIP-Seq Strategy to Identify Z-DNA Forming Sequences in the Human Genome.- Detection of Z-DNA Structures in Supercoiled Genome.- Thermogenomic Analysis of Left-Handed Z-DNA Propensities in Genomes.- DeepZ: A Deep-Learning Approach for Z-DNA Prediction.- Methods to Study Z-DNA-Induced Genetic Instability.- Single-Molecule Visualization of B-Z Transition in DNA Origami Using High-Speed AFM.- Adoption of A-Z Junctions in RNAs by Binding of Zα Domains.- Detecting Z-RNA in Mammalian Cells.- Identification of ADAR1 p150 and p110 Associated Edit Sites.- Z-DNA and Z-RNA: Methods Past and Future.